Your browser doesn't support javascript.
loading
A new Factor Xa inhibitor from Amblyomma cajennense with a unique domain composition
Batista, I F C; Ramos, O H P; Ventura, J S; Junqueira-de-Azevedo, I L M; Ho, P L; Chudzinski-Tavassi, A M.
Affiliation
  • Batista, I F C; Instituto Butantan. São Paulo. BR
  • Ramos, O H P; Instituto Butantan. São Paulo. BR
  • Ventura, J S; Instituto Butantan. São Paulo. BR
  • Junqueira-de-Azevedo, I L M; Instituto Butantan. São Paulo. BR
  • Ho, P L; Instituto Butantan. São Paulo. BR
  • Chudzinski-Tavassi, A M; Instituto Butantan. São Paulo. BR
Archives of Biochemistry and Biophysics ; 493(2): 151-156, 2010.
Article in English | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1059818
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
Bioactive compounds of great interest are found in the saliva of hematophagous organisms. While exploring a cDNA library derived from the salivary glands of the tick Amblyomma cajennense, a transcript that codes for a protein with unique structure (containing an N-terminal Kunitz-type domain and a C-terminus with no homology to any annotated sequences) was found. The recombinant mature form of this protein (¡­13.5 kDa) was produced in Escherichia coli BL21 (DE3), and it was able to inhibit Factor Xa (FXa) and extend global blood clotting times in vitro and ex vivo. Static and dynamic predictions of its tertiary structure indicate regions that may be related to its FXa inhibitor function.
Subject(s)
Search on Google
Add to My VHL
Print
XML
Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Anticoagulants Type of study: Prognostic study Limits: Animals Language: English Journal: Archives of Biochemistry and Biophysics Year: 2010 Document type: Article Institution/Affiliation country: Instituto Butantan/BR
Search on Google
Add to My VHL
Print
XML
Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Anticoagulants Type of study: Prognostic study Limits: Animals Language: English Journal: Archives of Biochemistry and Biophysics Year: 2010 Document type: Article Institution/Affiliation country: Instituto Butantan/BR