Modulation of eosinophil migration from bone marrow to lungs of allergic rats by nitric oxide
Biochemical Pharmacology
; 68(4): 631-639, 2004.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1060813
Responsible library:
BR78.1
Localization: BR78.1
ABSTRACT
Chronic blockade of nitric oxide (NO) synthesis attenuates the eosinophil infiltration into airways of allergic rats. This study was designed to investigate whether the inhibition of eosinophil influx to the lung of allergic rats reflects modifications in the pattern of cell mobilization from the bone marrow to peripheral blood and/or to lung. Male Wistar rats were treated with Nù-nitro-L-arginine methyl ester (L-NAME; 20 mg/rat per day) for 4 weeks and sensitized with ovalbumin (OVA). In control rats, the pulmonary OVA-challenge promoted an early (24 h) increase in the bone marrow eosinophil population that normalized at 48 h after OVA-challenge, at which time the eosinophils disappeared from the blood and reached the lungs in mass. In L-NAME-treated rats, an accumulation of eosinophils in bone marrow was observed at 24 and 48 h post-OVA-challenge. No variation in this cell type number was observed in peripheral blood and bronchoalveolar lavage throughout the time-course studied. In control rats, the adhesion of bone marrow eosinophils to fibronectin-covered wells was significantly increased at 24 h after OVA-challenge, whereas in L-NAME-treated rats the increased adhesion was detected at 48 h. A 32% decrease in the expression of inducible nitric oxide synthase (iNOS) (but not endothelial nitric oxide synthase; eNOS) in eosinophils from L-NAME-treated rats was observed. The levels of IgE, IgG1 and IgG2a were not affected by the L-NAME treatment. Our findings suggest that inhibition of NO synthesis upregulates the binding of eosinophils to extracellular matrix proteins such as fibronectin, producing a delayed efflux of eosinophils from bone marrow to peripheral blood and lungs.
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Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Main subject:
Eosinophils
/
Nitric Oxide
Limits:
Animals
Language:
English
Journal:
Biochemical Pharmacology
Year:
2004
Document type:
Article
Institution/Affiliation country:
Instituto Butantan/BR