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Microquantification of proteins with low chromophore content
Quintilio, W; Sato, R A; Silveira, A M da; Barratt, G; Fattal, E; Costa, M H Bueno da.
Affiliation
  • Quintilio, W; Instituto Butantan. São Paulo. BR
  • Sato, R A; Instituto Butantan. São Paulo. BR
  • Silveira, A M da; s.af
  • Barratt, G; s.af
  • Fattal, E; s.af
  • Costa, M H Bueno da; Instituto Butantan. São Paulo. BR
Biotechnology Techniques ; 11(9): 697-700, 1997.
Article in En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1060896
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
The advantages of the intrinsic fluorescence of the tryptophan and the absorbance of the methionine residues of the 18 kDa-hsp - a recombinant protein from Mycobacterium leprae - was exploited here to develop a sensitive and low costs method for protein assaying. They presented linearity between 3 and 1000 ìg of protein. The correlations between intrinsic fluorescence or absorbance at 230 nm and protein contents were both superiors to 0.99. These methods can be extended to others proteins with low aromatic residue contents
Subject(s)
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Collection: 06-national / BR Database: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Tryptophan / Methionine / Mycobacterium leprae Limits: Animals Language: En Journal: Biotechnology Techniques Year: 1997 Document type: Article
Search on Google
Collection: 06-national / BR Database: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Tryptophan / Methionine / Mycobacterium leprae Limits: Animals Language: En Journal: Biotechnology Techniques Year: 1997 Document type: Article