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Haemoglobin biosynthesis site in rabbit embryo erythroid cells
Cianciarullo, A M; Bertho, A L; Soares, M J; Hosoda, T M; Nogueira-Silva, S; Beçak, W.
Affiliation
  • Cianciarullo, A M; Instituto Butantan. São Paulo. BR
  • Bertho, A L; s.af
  • Soares, M J; s.af
  • Hosoda, T M; Instituto Butantan. São Paulo. BR
  • Nogueira-Silva, S; Instituto Butantan. São Paulo. BR
  • Beçak, W; Instituto Butantan. São Paulo. BR
Cell Biology International ; 27(9): 747-753, 2003.
Article in En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1061910
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, with erythroid cells obtained from rabbit embryos, in order to compare these results with those of animals treated with saponine or phenylhydrazine. Our results are similar to those obtained in assays with adult mammals, birds, amphibians, reptiles and fish, after induction of haemolytic anaemia. Therefore, the treatment did not interfere with the process studied, confirming our previous findings. Immunoelectron microscopy showed no labelling of mitochondria or other cellular organelles supposedly involved in the final biosynthesis of haemoglobin molecules, suggesting instead that it occurs free in the cytoplasm immediately after the liberation of haeme from the mitochondria, by electrostatic attraction between haeme and globin chains.
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Collection: 06-national / BR Database: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Hemoglobins / Erythroid Cells / Embryo, Mammalian Limits: Animals Language: En Journal: Cell Biology International Year: 2003 Document type: Article
Search on Google
Collection: 06-national / BR Database: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Hemoglobins / Erythroid Cells / Embryo, Mammalian Limits: Animals Language: En Journal: Cell Biology International Year: 2003 Document type: Article