Schistosoma mansoni Molecular characterization of Alkaline Phosphatase and expression patterns across life cycle stages
Experimental Parasitology
; 129(3): 284-291, Nov.2011.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1062792
Responsible library:
BR78.1
Localization: BR78.1
ABSTRACT
Here we describe the cloning and characterization of the Schistosoma mansoni Alkaline Phosphatase(SmAP), previously identified in the tegument of adult worms. SmAP encodes a complete sequence composedof 536 amino acids containing an N-terminal signal peptide, five N-glycosylation sites, and a GPIanchor signal, similar to that described for mammalian orthologs. Real-time RT-PCR and Western blotexperiments suggest a rapid translation as soon as cercariae are transformed into schistosomula. Immunolocalizationanalysis shows that the protein is widely distributed in the worm tissues, with increased concentration in the vitelline glands of female parasites. Furthermore, the surface localization of thisenzyme was quantitatively supported by its enzymatic activity in live ex vivo or cultured parasites throughout the life cycle stages. The fact that cercariae accumulate large amounts of SmAP mRNA, which rapidly translates into protein upon schistosomula transformation, indicates it may have an important role in host invasion.
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Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
Sec. Est. Saúde SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Main subject:
Schistosoma mansoni
/
Alkaline Phosphatase
/
Amino Acids
Limits:
Animals
Language:
English
Journal:
Experimental Parasitology
Year:
2011
Document type:
Article
Institution/Affiliation country:
Instituto Butantan/BR