Differentiation of african components of ancestry to stratify groups in a Casecontrol study of a brazilian urban population
Genetic Testing and Molecular Biomarkers
; 16(6): 524-530, 2012. tab, ilus
Article
in English
| Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP
| ID: biblio-1063089
Responsible library:
BR79.1
Localization: BR79.1
ABSTRACT
Background:
Balancing the subject composition of case and control groups to create homogenous ancestries between each group is essential for medical association studies.Methods:
We explored the applicability of single-tube 34-plex ancestry informative markers (AIM) single nucleotide polymorphisms (SNPs) to estimate the African Component of Ancestry (ACA) to design a future case-control association study of a Brazilian urban sample.Results:
One hundred eighty individuals (107 case group; 73 control group) self-described as white, brown-intermediate or black were selected. The proportions of the relative contribution of a variable number of ancestral population components were similar between case and control groups. Moreover, the case and control groups demonstrated similar distributions for ACA 0.50 categories. Notably a high number of outlier values (23 samples) were observed among individuals with ACA <0.25. These individuals presented a high probability of Native American and East Asian ancestral components; however, no individuals originally giving these self-described ancestries were observed in this study.Conclusions:
The strategy proposed for the assessment of ancestry and adjustment of case and control groups for an association study is an important step for the proper construction of the study, particularly when subjects are taken from a complex urban population. This can be achieved using a straight forward multiplexed AIM-SNPs assay of highly discriminatory ancestry markers.
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Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IDPCPROD
Main subject:
Polymorphism, Genetic
/
Urban Population
/
Genomics
Type of study:
Observational study
/
Risk factors
Aspects:
Social determinants of health
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Genetic Testing and Molecular Biomarkers
Year:
2012
Document type:
Article
Institution/Affiliation country:
Department of Biology, University of Aveiro/PT
/
Faculty of Pharmaceutical Sciences, University of Sao Paulo/BR
/
Instituto Dante Pazzanese de Cardiologia/BR
/
University Hospital, University of Sao Paulo/BR
/
University of Santiago de Compostela/ES