Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
Int. j. nanomed. (Online)
; 5: 203-211, 2010.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1063573
Responsible library:
BR78.1
Localization: BR78.1
ABSTRACT
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administeredin biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the eliminationand biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysisof the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carriedout in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique forthe SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).
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Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Main subject:
Magnetic Resonance Spectroscopy
/
Nanoparticles
Limits:
Animals
Language:
English
Journal:
Int. j. nanomed. (Online)
Year:
2010
Document type:
Article
Institution/Affiliation country:
Instituto Butantan/BR