Flow cytometry assay for intracellular rabies virus detection
J Virol Meth
; 105: 181-186, 2002.
Article
in Portuguese
| Sec. Est. Saúde SP, SESSP-IPPROD, Sec. Est. Saúde SP
| ID: biblio-1063717
Responsible library:
BR84.1
Localization: S3146
RESUMO
Following previous studies reporting microbiological diagnosis by flow cytometry, the possibility of using this method was examined to monitor infection of susceptible cell lines by a fixed rabies virus strain (Pasteur Virus strainPV) or a wild rabies virus strain (WRS). Suspensions of BHK-21 and C6 cells were infected with viruses and a time course of virus infection was established. Sequentially, at several time points, infected and control uninfected cells were fixed, permeabilized, and stained with a rabies virus-specific antibody conjugate. This was achieved by resuspending cells in a solution containing p-formaldehyde in FACS lysis fluid, which allowed the detection of intracellular virus with flourescein-coupled antibodies by flow cytometry. A Becton Dickinson FACSCalibur® flow cytometer was used to analyze the percentage of cells infected and the kinetics of the infection process was determined. As early as 12 h after inoculation with both rabies virus strains, significant levels (P<0.01) of infection (from 4.7 to 7.1%) were detected by flow cytometry. The maximum level of infection was obtained at 48 h in C6 cells (88%) with both viruses. The advantages of this method for examination of intracellular virus infection are discussed.
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Collection:
National databases
/
Brazil
Health context:
SDG3 - Health and Well-Being
/
Neglected Diseases
Health problem:
Target 3.3: End transmission of communicable diseases
/
Neglected Diseases
/
Zoonoses
Database:
Sec. Est. Saúde SP
/
SESSP-IPPROD
Main subject:
Rabies
/
Rabies virus
/
Flow Cytometry
Type of study:
Diagnostic study
Language:
Portuguese
Journal:
J Virol Meth
Year:
2002
Document type:
Article
Institution/Affiliation country:
Secretaria de Estado da Saúde de São Paulo/BR