Multiparametric analyses of hybridoma growth on glass cylinders in a packed-bed bioreactor system with internal aeration. Serum-supplemented and serum-free media comparison for MAb production

ABSTRACT

Monoclonal antibodies are one of the most important products of biotechnology and laboratories and companies all over the world are pursuing their large-scale production. Herein we report a protocol for hybridoma cell cultivation over small glass cylinders inside a 3 liter bioreactor vessel which leads to the production and purification - in order of grams - of one MAb intended for human therapeutic use. This protocol proved to be simple,reproducible and cost effective. Three trials are reported the first two using conventionally serum-supplementedmedium culture and producing 3.15 and 2.1 g of purified MAb in 30 and 21 days respectively, and the third oneusing serum-free medium culture and producing 6 g of purified MAb in 36 days. We have ascertained the stability of the hybridoma by its cloning directly in serum-free medium. The downstream processing of the serum-free trial wasdone in a single step, concentrating large volumes of supernatant while simultaneously purifying the antibody.