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Large unilamellar vesicles as trehalose-stabilised vehicles forvaccines storage time and in vivo studies
Quintilio, W; Sato, R A; Sant’Anna, OA; Esteves, M I; Sesso, A; de Araujo, PS; da Costa, MH Bueno.
Affiliation
  • Quintilio, W; Instituto Butantan. São Paulo. BR
  • Sato, R A; Instituto Butantan. São Paulo. BR
  • Sant’Anna, OA; Instituto Butantan. São Paulo. BR
  • Esteves, M I; Instituto Butantan. São Paulo. BR
  • Sesso, A; s.af
  • de Araujo, PS; s.af
  • da Costa, MH Bueno; Instituto Butantan. São Paulo. BR
Journal of Controlled Release ; 67(2-3): 409-413, 2000.
Article in En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1064230
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
Liposomes, as a pharmaceutical formulation must display a long shelf life. The recombinant heat-shock protein fromMycobacterium leprae (18-kDa hsp) or its N-acylated derivative, when entrapped within or externally associated with largeunilamellar vesicles, acts as a T-epitope source. Freeze-fracture electron microscopy shows unequivocally that trehaloseavoids aggregation and fusion of these vesicles. Formulations containing trehalose retained up to 98% of the entrappedprotein. The highest antibody level is obtained with formulations containing trehalose. The adjuvant effect depends on theliposomal membrane integrity.
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Collection: 06-national / BR Database: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Vaccines Limits: Animals / Humans Language: En Journal: Journal of Controlled Release Year: 2000 Document type: Article
Search on Google
Collection: 06-national / BR Database: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Vaccines Limits: Animals / Humans Language: En Journal: Journal of Controlled Release Year: 2000 Document type: Article