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Purification of capsular polysaccharide produced by Haemophilus influenzae type b through a simple, efficient and suitable method for scale-up
Takagi, Mickie; Lima, Rodrigo Barbosa; Albani, Silvia Maria Ferreira; Zangirolami, Teresa Cristina; Tanizaki, Martha Massako; Crespo, Joaquin Cabrera.
Affiliation
  • Takagi, Mickie; Instituto Butantan. São Paulo. BR
  • Lima, Rodrigo Barbosa; Instituto Butantan. São Paulo. BR
  • Albani, Silvia Maria Ferreira; Instituto Butantan. São Paulo. BR
  • Zangirolami, Teresa Cristina; s.af
  • Tanizaki, Martha Massako; Instituto Butantan. São Paulo. BR
  • Crespo, Joaquin Cabrera; Instituto Butantan. São Paulo. BR
Article in English | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1064265
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
Haemophilus inXuenzae type b, an encapsulatedbacterium, causes meningitis in infants worldwide. The capsularpolysaccharide conjugated to a carrier protein is eVectivein the prevention of such infections. The traditional puriWcationprocess of polysaccharide from bacterial cultures for vaccineproduction is based on several selective precipitationswith solvents such as ethanol, phenol, and cationic detergents.The separations of solid and liquid phases are based oncontinuous centrifugation in explosion proof installations. Thelipopolysaccharides are separated by ultracentrifugation. Asimple and eYcient method that can easily be scaled-up wasdeveloped for puriWcation of polysaccharides. The ethanolprecipitation was reduced to only two steps. The phenol treatmentwas substituted by ultraWltration and enzymatic digestion.Lipopolysaccharide was removed by ultraWltrationtogether with addition of detergent and chelating agent.
Subject(s)
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Collection: National databases / Brazil Health context: Neglected Diseases / SDG3 - Target 3.3 End transmission of communicable diseases Health problem: Zoonoses / Meningitis Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Haemophilus influenzae type b / Meningococcal Vaccines / Meningitis, Haemophilus Limits: Child / Female / Humans / Male Language: English Journal: Journal of Industrial Microbiology and Biotechnology Year: 2008 Document type: Article Institution/Affiliation country: Instituto Butantan/BR
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Collection: National databases / Brazil Health context: Neglected Diseases / SDG3 - Target 3.3 End transmission of communicable diseases Health problem: Zoonoses / Meningitis Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Haemophilus influenzae type b / Meningococcal Vaccines / Meningitis, Haemophilus Limits: Child / Female / Humans / Male Language: English Journal: Journal of Industrial Microbiology and Biotechnology Year: 2008 Document type: Article Institution/Affiliation country: Instituto Butantan/BR
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