Purification of capsular polysaccharide produced by Haemophilus influenzae type b through a simple, efficient and suitable method for scale-up
Journal of Industrial Microbiology and Biotechnology
; 35(11): 1217-1222, 2008.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1064265
Responsible library:
BR78.1
Localization: BR78.1
ABSTRACT
Haemophilus inXuenzae type b, an encapsulatedbacterium, causes meningitis in infants worldwide. The capsularpolysaccharide conjugated to a carrier protein is eVectivein the prevention of such infections. The traditional puriWcationprocess of polysaccharide from bacterial cultures for vaccineproduction is based on several selective precipitationswith solvents such as ethanol, phenol, and cationic detergents.The separations of solid and liquid phases are based oncontinuous centrifugation in explosion proof installations. Thelipopolysaccharides are separated by ultracentrifugation. Asimple and eYcient method that can easily be scaled-up wasdeveloped for puriWcation of polysaccharides. The ethanolprecipitation was reduced to only two steps. The phenol treatmentwas substituted by ultraWltration and enzymatic digestion.Lipopolysaccharide was removed by ultraWltrationtogether with addition of detergent and chelating agent.
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Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
/
SDG3 - Target 3.3 End transmission of communicable diseases
Health problem:
Zoonoses
/
Meningitis
Database:
Sec. Est. Saúde SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Main subject:
Haemophilus influenzae type b
/
Meningococcal Vaccines
/
Meningitis, Haemophilus
Limits:
Child
/
Female
/
Humans
/
Male
Language:
English
Journal:
Journal of Industrial Microbiology and Biotechnology
Year:
2008
Document type:
Article
Institution/Affiliation country:
Instituto Butantan/BR