Your browser doesn't support javascript.
loading
Transfer of arachidonic acid from lymphocytes to macrophages
Peres, C M; Sampaio, S C; Cury, Y; Newsholme, P; Curi, R.
Affiliation
  • Peres, C M; s.af
  • Sampaio, S C; Instituto Butantan. São Paulo. BR
  • Cury, Y; Instituto Butantan. São Paulo. BR
  • Newsholme, P; s.af
  • Curi, R; s.af
Lipids ; 38(6): 633-639, 2003.
Article in English | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1064629
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
The incorporation and oxidation of arachidonic acid (AA) by rat lymphocytes (LY), the transfer of AA from LY to rat macrophages (Mö) in co-culture, and the subsequent functional impact on Mö phagocytosis were investigated. The rate of incorporation of [1-14C]AA by untreated-LY and TG (thioglycolate treated)-LY (TG-LY) was 158 ± 8 nmol/1010 LY per h for both untreated-LY and TG-LY. The oxidation of AA was 3.4-fold higher in TG-LY as compared with untreated cells. LY from TG-injected rats had a 2.5-fold increase in the oxidation of palmitic (PA), oleic (OA), and linoleic (LA) acids. After 6 h of incubation, [14C] from AA was distributed mainly into phospholipids. The rate of incorporation into total lipids was 1071 nmol/1010 cells in untreated-LY and 636 nmol/1010 cells in TG-LY. [14C]AA was transferred from LY to co-cultured Mö in substantial amounts (8.7 nmol for untreated and 15 nmol per 1010 for TG cells). Exogenously added AA, PA, OA, and LA caused a significant reduction of phagocytosis by resident cells. Mö co-cultured with AA-preloaded LY showed a significant reduction of the phagocytic capacity (about 40% at 35 ìM). LY preloaded with PA, LA, and OA also induced a reduction in phagocytic capacity of co-cultured Mö. TG treatment abolished the AA-induced inhibition of phagocytosis in Mö co-cultured with TG-LY. Therefore, the transfer of AA between leukocytes is a modulated process and may play an important role in controlling inflammatory and immune response.
Subject(s)
Search on Google
Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Lymphocytes / Arachidonic Acid Limits: Animals Language: English Journal: Lipids Year: 2003 Document type: Article Institution/Affiliation country: Instituto Butantan/BR
Search on Google
Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Lymphocytes / Arachidonic Acid Limits: Animals Language: English Journal: Lipids Year: 2003 Document type: Article Institution/Affiliation country: Instituto Butantan/BR
...