Transfer of arachidonic acid from lymphocytes to macrophages
Lipids
; 38(6): 633-639, 2003.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1064629
Responsible library:
BR78.1
Localization: BR78.1
ABSTRACT
The incorporation and oxidation of arachidonic acid (AA) by rat lymphocytes (LY), the transfer of AA from LY to rat macrophages (Mö) in co-culture, and the subsequent functional impact on Mö phagocytosis were investigated. The rate of incorporation of [1-14C]AA by untreated-LY and TG (thioglycolate treated)-LY (TG-LY) was 158 ± 8 nmol/1010 LY per h for both untreated-LY and TG-LY. The oxidation of AA was 3.4-fold higher in TG-LY as compared with untreated cells. LY from TG-injected rats had a 2.5-fold increase in the oxidation of palmitic (PA), oleic (OA), and linoleic (LA) acids. After 6 h of incubation, [14C] from AA was distributed mainly into phospholipids. The rate of incorporation into total lipids was 1071 nmol/1010 cells in untreated-LY and 636 nmol/1010 cells in TG-LY. [14C]AA was transferred from LY to co-cultured Mö in substantial amounts (8.7 nmol for untreated and 15 nmol per 1010 for TG cells). Exogenously added AA, PA, OA, and LA caused a significant reduction of phagocytosis by resident cells. Mö co-cultured with AA-preloaded LY showed a significant reduction of the phagocytic capacity (about 40% at 35 ìM). LY preloaded with PA, LA, and OA also induced a reduction in phagocytic capacity of co-cultured Mö. TG treatment abolished the AA-induced inhibition of phagocytosis in Mö co-cultured with TG-LY. Therefore, the transfer of AA between leukocytes is a modulated process and may play an important role in controlling inflammatory and immune response.
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Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Main subject:
Lymphocytes
/
Arachidonic Acid
Limits:
Animals
Language:
English
Journal:
Lipids
Year:
2003
Document type:
Article
Institution/Affiliation country:
Instituto Butantan/BR