Genetic and non-genetic factors that increase the risk of non-syndromic cleft lip and/or palate development
Oral Diseases
; 21(3): 393-399, 2015.
Article
in English
| Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP
| ID: biblio-1065039
Responsible library:
BR79.1
Localization: BR79.1
ABSTRACT
ObjectivesWe investigated the relationship between non-syndromic cleft lip/palate (NSCLP) and polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MTR), methionine synthase reductase (MTRR), and RFC1, as well as the corresponding interactions with environmental factors.Subjects and MethodsOne hundred and forty NSCLP patients and their mothers, as well as 175 control individuals and their mothers, were recruited. Information regarding smoking and alcohol consumption was recorded. Blood samples were obtained in order to measure serum folate and cobalamin, as well as, plasma total homocysteine concentrations and to extract DNA. Polymorphisms in MTHFR(677C>T and 1298A>C), MTR(2756A>G), MTR(66A>G), and RFC1(80A>G) were analyzed by PCRrestriction fragment length polymorphism.ResultsAmong the patients, 59.5% had cleft lip and palate, 22.0% had cleft palate, and 18.5% had cleft lip only. Maternal alcohol consumption and reduced folic acid concentrations in both children and mothers (P < 0.001 and P = 0.003, respectively) were risk factors for NSCLP. Patients and their mothers carrying the MTHFR 667T allele showed lower serum folate than CC (P = 0.011 and P = 0.030, respectively). Mothers who carried the MTHFR 1298C allele exhibited increased risk of having a child with NSCLP, after adjusting for alcohol consumption (OR 1.75, 95% CI 1.032.99, P = 0.038).ConclusionsReduced folic acid levels, alcohol consumption, and the MTHFR 677T and 1298C alleles may have contributed to NSCLP development in this sample population from Rio Grande do Norte.
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Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IDPCPROD
Main subject:
Palate
/
Alcohol Drinking
/
Folic Acid
Type of study:
Etiology study
/
Risk factors
Language:
English
Journal:
Oral Diseases
Year:
2015
Document type:
Article
Institution/Affiliation country:
Federal University of Rio Grande do Norte/BR
/
M das G Almeida1, Department of Federal University of Rio Grande do Norte/BR
/
School of Pharmaceutical Sciences, University of São Paulo/BR
/
Universidade CEUMA/BR