Eumenitin, a novel antimicrobial peptide from the venom of the solitary eumenine wasp Eumenes rubronotatus
Peptides
; 27(11): 2624-2631, 2006.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1065265
Responsible library:
BR78.1
Localization: BR78.1
ABSTRACT
A novel antimicrobial peptide, eumenitin, was isolated from the venom of the solitary eumenine wasp Eumenes rubronotatus. The sequence of eumenitin, LeuAsnLeuLysGlyIlePheLysLysValAlaSerLeuLeuThr, was mostly analyzed by mass spectrometry together with Edman degradation, and corroborated by solid-phase synthesis. This peptide has characteristic features of cationic linear á-helical antimicrobial peptides, and therefore, can be predicted to adopt an amphipathic á-helix secondary structure. In fact, the CD spectra of eumenitin in the presence of TFE or SDS showed a high content of á-helical conformation. Eumenitin exhibited inhibitory activity against both Gram-positive and Gram-negative bacteria, and moderately stimulated degranulation from the rat peritoneal mast cells and the RBL-2H3 cells, but showed no hemolytic activity against human erythrocytes. This antimicrobial peptide in the eumenine wasp venom may play a role in preventing potential infection by microorganisms during prey consumption by their larvae.
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Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Zoonoses
Database:
Sec. Est. Saúde SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Main subject:
Wasps
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Peptides
Year:
2006
Document type:
Article
Institution/Affiliation country:
Instituto Butantan/BR