Bacteroides Fragilis induces conformational alterations in Trichomonas Vaginalis ultrastructure in an in vitro interaction
Rev. patol. trop
; 49(1): 11-20, 2020. ilus, graf
Article
in English
| LILACS
| ID: biblio-1099696
Responsible library:
BR15.1
ABSTRACT
Bacteroides genus are commonly found on mucous membranes, including the female genital tract, acting as agents for several site infections. Anaerobic infections are usually polymicrobial and endogenous. Trichomonas vaginalis, the trichomoniasis etiologic agent, is a facultative anaerobic flagellated parasite spread worldwide. The purpose of this study was to explore the association between vaginal bacteria and T. vaginalis, as well as to understand factors that may favour the infection of T. vaginalis. We have, therefore, used T. vaginalis trophozoites and the species Bacteroides fragilis, which is considered the most important in its genus, once it is the most commonly isolated bacteria from endogenous infections. The parasite-bacteria interaction was performed in different proportions in periods varying from 1 to 12 hours applying viability tests. The data were analyzed to compare the parasite viability in vitro in the presence and absence of B. fragilis. The results indicate that in the 1100 proportion postinteraction analysis, ultrastructural alterations were noticeable after 6 hours. After 8 hours, T. vaginalis viability decreased, and after 12 hours of interaction no viable trophozoites were found. These data suggest that the parasite can deal with B. fragilis in short interaction periods. However, in longer interaction periods the trophozoites collapse, indicating that B. fragilis may produce toxic metabolites against T. vaginalis activity.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Bacteroides fragilis
/
Trichomonas vaginalis
/
In Vitro Techniques
/
Vaginosis, Bacterial
/
Genital Diseases, Female
Language:
English
Journal:
Rev. patol. trop
Journal subject:
Tropical Medicine
/
Pathology
Year:
2020
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro Universitário Estadual da Zona Oeste/BR
/
Instituto Butantã/BR
/
Universidade Estadual do Norte Fluminense Darcy Ribeiro/BR