Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication
Acta cir. bras
; 35(3): e202000301, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1130626
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea.Methods:
Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue was performed.Results:
Statistically significant differences were seen in liver, brain and spinal cord TBARS levels.Conclusions:
Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Spinal Cord
/
Brain
/
Oxidative Stress
/
Hydroxyurea
/
Anemia, Sickle Cell
/
Liver
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2020
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Laboratory of Experimental Carcinogenicity/BR
/
UFMS/BR
/
Universidade Federal do Mato Grosso do Sul/BR
/
Universidade de São Paulo/BR