Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
Acta cir. bras
; 35(6): e202000605, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1130648
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE 30 min preoperatively; POST 30 min postoperatively; PP 30 min pre- and postoperatively; C control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Pneumoperitoneum
/
Laparoscopy
/
Surgical Wound
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2020
Document type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Centro Universitário de Brasília/BR
/
Irmandade da Santa Casa de Misericórdia de São Paulo/BR
/
Johns Hopkins Medicine/US
/
UnB/BR
/
UniCEUB/BR
/
Universidade Federal de Goiás/BR
/
Universidade de Brasília/BR