Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study
Braz. arch. biol. technol
; 63: e20190179, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1132181
Responsible library:
BR1.1
ABSTRACT
Abstract (1) Background:
The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2)Methods:
To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3)Results:
The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4)Conclusion:
Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
Full text:
Available
Collection:
International databases
Health context:
SDG3 - Health and Well-Being
/
Neglected Diseases
Health problem:
Target 3.3: End transmission of communicable diseases
/
Neglected Diseases
/
Tuberculosis
Database:
LILACS
Main subject:
Tuberculosis, Multidrug-Resistant
/
Antibiotics, Antitubercular
/
Mycobacterium tuberculosis
Type of study:
Controlled clinical trial
/
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2020
Document type:
Article
Affiliation country:
Brazil
/
Portugal
Institution/Affiliation country:
Adolfo Lutz Institute/BR
/
Federal University of Grande Dourados/BR
/
Federal University of Minas Gerais/BR
/
Federal University of Rio Grande/BR
/
Federal University of Rio de Janeiro/BR
/
NOVA University of Lisbon/PT
/
Oswaldo Cruz Foundation/BR
/
Sergio Arouca National Public Health School/BR
/
State Secretariat of Health of Rio Grande do Sul/BR
/
Tropical Medicine Foundation Dr. Heitor Vieira Dourado/BR