Susceptibilities of Mycobacterium leprae and M. avium complex to the H202-Fe-mediated halogenation system supplemented with antimicrobial agents
Int. j. lepr. other mycobact. dis
; 57(3): 628-632, sept. 1989. tab
Article
in English
| Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1226447
Responsible library:
BR191.1
Localization: [{"text": "Br191.1"}]
ABSTRACT
The susceptibilities of Mycobacterium leprae and M. avium complex (MAC) to the H2-O2-Fe-mediated halogenation system supplemented with antimicrobial agents were evaluated by fluorescein diacetate-ethidium bromide (FDA/EB) staining. In the case of M. leprae, the number of greenstained bacteria (intact cells) was reduced in the presence of the H2O2-Fe-mediated halogenation system supplemented with agents possessing antileprosy activity, such as rifampin, 4,4'-diaminodiphenylsulfone (dapsone), clofazimine, and ofloxacin. In the case of the MAC strain, although viable units of the organisms were reduced by the halogenation system alone, the number of greenstained cells in the FDA/EB stain was not reduced, even when the halogenation system was used in combination with ofloxacin. Because stainability of the cells is related to structural and functional intactness of the membrane, differences between M. leprae and the MAC strain imply possible differences in the rigidity of the cell membrane
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Leprosy
/
Neglected Diseases
Database:
HANSEN
/
Hanseníase Leprosy
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
Main subject:
Mycobacterium avium
/
Mycobacterium leprae
Language:
English
Journal:
Int. j. lepr. other mycobact. dis
Year:
1989
Document type:
Article
Institution/Affiliation country:
Shimane Medical University/JP
/
Shiname Medical University/JP
/
Shiname Medical Univesity/JP