Identification fo Mycobacterium leprae antigens in tissues of leprosy patients using monoclonal antibodies
Int. j. lepr. other mycobact. dis
; 57(3): 652-658, sept. 1989. tab, ilus
Article
in English
| Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1226451
Responsible library:
BR191.1
Localization: [{"text": "Br191.1"}]
ABSTRACT
Five monoclonal antibodies (MAbs) directed against antigens of Mycobacterium leprae were tested for their ability to bind to components of tissue sections prepared from biopsies taken from patients with various forms of leprosy. Immunoperoxidase was the most successful marker system used, although immunofluorescence and alkaline phosphatase were also successful in certain cases. Positivity was high with all five antibodies successfully staining those sections containing a bacterial index of 3+ or more; sections with 0 bacterial counts also had areas staining positively with two of the MAbs. The positive staining in the tissues was confined to areas infiltrated by inflammatory cells; however it was not identifiable as being associated with individual bacteria. These findings suggest that immunostaining with specific monoclonal antibodies can help to identify leprosy in diagnostic samples in which acid-fast bacilli are not identifiable by standard histochemical means. Immunohistochemical techniques are likely to be valuable in studies of the distribution of M. leprae antigens and their association with individual tissue elements
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Leprosy
/
Neglected Diseases
Database:
HANSEN
/
Hanseníase Leprosy
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
Main subject:
Antibodies, Monoclonal
/
Mycobacterium leprae
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Int. j. lepr. other mycobact. dis
Year:
1989
Document type:
Article
Institution/Affiliation country:
Central Jalma Institute for Leprosy/IN