Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR
s.l; s.n; 2003. 6 p. ilus, tab.
Non-conventional
in En
| SES-SP, HANSEN, HANSENIASE, SESSP-ILSLACERVO, SES-SP
| ID: biblio-1241184
Responsible library:
BR191.1
Localization: [{"text": "BR191.1", "_a": "09096/s"}]
ABSTRACT
The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively.
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Collection:
06-national
/
BR
Database:
HANSEN
/
HANSENIASE
/
SES-SP
/
SESSP-ILSLACERVO
Main subject:
Polymorphism, Genetic
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Point Mutation
/
Polymorphism, Single-Stranded Conformational
/
Drug Resistance, Multiple, Bacterial
/
Korea
/
Leprostatic Agents
/
Leprosy
Type of study:
Diagnostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Year:
2003
Document type:
Non-conventional