Determination of clofazimine in leprosy patients by high-performance liquid chromatography
s.l; s.n; 2003. 4 p. ilus, tab.
Non-conventional
in English
| Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1241393
Responsible library:
BR191.1
Localization: [{"text": "BR191.1", "_a": "01158/s"}]
ABSTRACT
An original, simple, specific, and rapid high-performance liquid chromatography assay for the determination of clofazimine in human plasma is presented. The procedure consists of extracting the drug and the internal standard (medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (11, v/v) at pH 3.0, after precipitating the proteins with methanol. The drugs were then quantitated on a reversed-phase C8 using a mobile phase consisting of a mixture of methanol/0.25 N sodium acetate buffer at pH 3.0 (7426, v/v). The flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The precision, linearity, and limit of quantitation of the method were within acceptable limits. The method was considered adequate and could be applied in studies involving blood level monitoring and pharmacokinetics in leprosy patients.
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Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Leprosy
/
Neglected Diseases
Database:
HANSEN
/
Hanseníase Leprosy
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
Main subject:
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Clofazimine
/
Drug Monitoring
/
Leprostatic Agents
/
Leprosy
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Language:
English
Year:
2003
Document type:
Non-conventional