Microsatellite mapping of Mycobacterium leprae populations in infected humans
s.l; s.n; Nov. 2004. 6 p. ilus, map, tab.
Non-conventional
in English
| Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1241688
Responsible library:
BR191.1
Localization: [{"text": "BR191.1", "_a": "09333/s"}]
ABSTRACT
To investigate genetic diversity in a bacterial population, we measured the copy numbers of simple sequence repeats, or microsatellites, in Mycobacterium leprae from patients living in and around Hyderabad, India. Three microsatellite loci containing trinucleotide or dinucleotide repeats were amplified from infected tissues, and the copy numbers were established by sequence analysis. Extensive diversity was observed in a cross-sectional survey of 33 patients, but closely related profiles were found for members of a multicase family likely to share a common transmission source. Sampling of multiple tissues from single individuals demonstrated identical microsatellite profiles in the skin, nasal cavity, and bloodstream but revealed differences at one or more loci for M. leprae present in nerves. Microsatellite mapping of M. leprae represents a useful tool for tracking short transmission chains. Comparison of skin and nerve lesions suggests that the evolution of disease within an individual involves the expansion of multiple distinct subpopulations of M. leprae.
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Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Leprosy
/
Neglected Diseases
Database:
HANSEN
/
Hanseníase Leprosy
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
Main subject:
Species Specificity
/
Genetic Variation
/
Family
/
Polymerase Chain Reaction
/
Cross-Sectional Studies
/
Bacterial Typing Techniques
/
Gene Dosage
/
Microsatellite Repeats
/
Leprosy
/
Mycobacterium leprae
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Limits:
Female
/
Humans
/
Male
Language:
English
Year:
2004
Document type:
Non-conventional