Vibrio sp. ArtGut-C1, a polyhydroxybutyrate producer isolated from the gut of the aquaculture live diet Artemia (Crustacea)
Electron J Biotechnol
; 49: 22-28, Jan. 2021. ilus, graf, tab
Article
in English
| LILACS
| ID: biblio-1291938
Responsible library:
CL1.1
ABSTRACT
BACKGROUND:
Vibrio species display variable and plastic fitness strategies to survive and interact with multiple hosts, including marine aquaculture species that are severely affected by pathogenic Vibrios. The culturable Vibrio sp. strain ArtGut-C1, the focus of this study, provides new evidence of such phenotypic plasticity as it accumulates polyhydroxybutyrate (PHB), a biodegradable polymer with anti-pathogen activity, particularly in the marine larviculture phase. The strain was isolated from the gut of laboratory-reared Artemia individuals, the live diet and PHB carrier used in larviculture. Its main phenotypic properties, taxonomic status and genomic properties are reported based on the whole-genome sequencing.RESULTS:
Vibrio sp. ArtGut-C1 yielded 72.6% PHB of cells' dry weight at 25 C. The genomic average nucleotide identity (ANI) shows it is closely related to V. diabolicus (ANI 88.6%). Its genome contains 5,236,997- bp with 44.8% GC content, 3,710 protein-coding sequences, 96 RNA, 9 PHB genes functionally related to PHB metabolic pathways, and several genes linked to competing and colonizing abilities.CONCLUSIONS:
This culturable PHB-accumulating Vibrio strain shows high genomic and phenotypic variability. It may be used as a natural pathogen biocontrol in the marine hatchery and as a potential cell factory for PHB production.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Zoonoses
Database:
LILACS
Main subject:
Artemia
/
Vibrio
/
Polyhydroxyalkanoates
/
Hydroxybutyrates
Limits:
Animals
Language:
English
Journal:
Electron J Biotechnol
Year:
2021
Document type:
Article
Affiliation country:
Chile
/
Japan
Institution/Affiliation country:
Hiroshima University/JP
/
Universidad de La Frontera/CL
/
Universidad de Los Lagos/CL
/
Universidad de Los Lagos/KY