Serological Testing for COVID-19, Immunological Surveillance, and Exploration of Protective Antibodies
Front. immunol
; 12: 1-10, 2021. graf, tab
Article
in English
| CONASS, Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP
| ID: biblio-1292830
Responsible library:
BR79.1
ABSTRACT
Serological testing is a powerful tool in epidemiological studies for understanding viral circulation and assessing the effectiveness of virus control measures, as is the case of SARS-CoV-2, the pathogenic agent of COVID-19. Immunoassays can quantitatively reveal the concentration of antiviral antibodies. The assessment of antiviral antibody titers may provide information on virus exposure, and changes in IgG levels are also indicative of a reduction in viral circulation. In this work, we describe a serological study for the evaluation of antiviral IgG and IgM antibodies and their correlation with antiviral activity. The serological assay for IgG detection used two SARS-CoV-2 proteins as antigens, the nucleocapsid N protein and the 3CL protease. Cross-reactivity tests in animals have shown high selectivity for detection of antiviral antibodies, using both the N and 3CL antigens. Using samples of human serum from individuals previously diagnosed by PCR for COVID-19, we observed high sensitivity of the ELISA assay. Serological results with human samples also suggest that the combination of higher titers of antiviral IgG antibodies to different antigen targets may be associated with greater neutralization activity, which can be enhanced in the presence of antiviral IgM antibodies.
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
SDG3 - Target 3.3 End transmission of communicable diseases
Health problem:
Pneumonia
Database:
CONASS
/
Sec. Est. Saúde SP
/
SESSP-IDPCPROD
Main subject:
Immunoassay
/
Nucleocapsid
/
Coronavirus 3C Proteases
/
SARS-CoV-2
/
COVID-19
Type of study:
Screening study
Language:
English
Journal:
Front. immunol
Year:
2021
Document type:
Article
Institution/Affiliation country:
Brazilian Biosciences National Laboratory, Center for Research in Energy and Materials/BR
/
Infectious Diseases Section and Hospital Infection Control Committee, Dante Pazzanese Cardiology Institute/BR
/
Laboratory of Emerging Viruses (LEVE), Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas/BR
/
Medical School, University of Campinas/BR
/
Molecular Sciences Undergrad Program, University of São Paulo/BR
/
Research Division, Dante Pazzanese Cardiology Institute/BR