CCR6 expression reduces mouse survival upon malarial challenge with Plasmodium berghei NK65 strain
Mem. Inst. Oswaldo Cruz
; 117: e210287, 2022. graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1386359
Responsible library:
BR1.1
ABSTRACT
BACKGROUND It has been demonstrated that proteins expressed by liver-stage Plasmodium parasites can inhibit the translocation of transcription factors to the nucleus of different cells. This process would hinder the expression of immune genes, such as the CCL20 chemokine. OBJECTIVE Since CCR6 is the only cognate receptor for CCL20, we investigated the importance of this chemokine-receptor axis against rodent malaria. METHODS CCR6-deficient (KO) and wild-type (WT) C57BL/6 mice were challenged with Plasmodium berghei (Pb) NK65 sporozoites or infected red blood cells (iRBCs). Liver parasitic cDNA, parasitemia and serum cytokine concentrations were respectively evaluated through reverse transcription-polymerase chain reaction (RT-PCR), staining thin-blood smears with Giemsa solution, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Although the sporozoite challenges yielded similar liver parasitic cDNA and parasitemia, KO mice presented a prolonged survival than WT mice. After iRBC challenges, KO mice kept displaying higher survival rates as well as a decreased IL-12 p70 concentration in the serum than WT mice. CONCLUSION Our data suggest that malaria triggered by PbNK65 liver- or blood-stage forms elicit a pro-inflammatory environment that culminates with a decreased survival of infected C57BL/6 mice.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Malaria
Database:
LILACS
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2022
Document type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
New York University School of Medicine/US
/
Universidade Federal de Santa Catarina/BR
/
Universidade de São Paulo/BR