Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil
Rev. Soc. Bras. Med. Trop
; 55: e0613, 2022. tab
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1387533
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Background:
In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laboratory techniques.Methods:
In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular techniques.Results:
To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M. peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49).Conclusions:
Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the application of standardized criteria, such as those set forth by the ATS.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Tuberculosis
Database:
LILACS
Type of study:
Observational study
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Prognostic study
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Risk factors
Country/Region as subject:
South America
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Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2022
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz/BR
/
Fundação de Medicina Tropical Dr. Heitor Vieira Dourado/BR
/
Secretaria de Estado da Saúde do Rio Grande do Sul/BR
/
Universidade Federal de Minas Gerais/BR
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Universidade Federal de Ouro Preto/BR
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Universidade Federal do Rio de Janeiro/BR
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Universidade Luterana do Brasil/BR
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Universidade do Estado do Amazonas/BR