Humoral response to spike S1 and S2 and nucleocapsidproteins on microarray after SARSCoV2 infection
J. med. virol
; 94(1): 178-185, 2022. tab, graf
Article
in English
| Coleciona SUS, Sec. Est. Saúde SP, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO
| ID: biblio-1393242
Responsible library:
BR91.2
Localization: BR76.1; Digital
ABSTRACT
Many aspects of the humoral immune response to severe acute respiratory syn-drome coronavirus 2 (SARSCoV2), such as its role in protection after natural in-fection, are still unclear. We evaluated IgA and IgG response to spike subunits 1 and2 (S1 and S2) and Nucleocapsid proteins of SARSCOV2 in serum samples of 109volunteers with viral RNA detected or seroconversion with different clinical evolu-tion (asymptomatic, mild, moderate, and severe coronavirus disease 2019), using theViraChip®Test Kit. We observed that the quantification of antibodies to all antigenshad a positive correlation to disease severity, which was strongly associated with thepresence of comorbidities. Seroreversion was not uncommon even during the short(median of 77 days) observation, occurring in 15% of mildasymptomatic cases at amedian of 55 days for IgG and 46 days for IgA. The time to reach the maximalantibody response did not differ significantly among recovered and deceased vo-lunteers. Our study illustrated the dynamic of antiS1, antiN, and antiS2 IgA andIgG antibodies, and suggests that high production of IgG and IgA does not guaranteeprotection to disease severity and that functional responses that have been studiedby other groups, such as antibody avidity, need further attention. (AU)
Full text:
Available
Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IALACERVO
/
SESSP-IALPROD
/
Coleciona SUS
Main subject:
Nucleocapsid
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Protein Array Analysis
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Spike Glycoprotein, Coronavirus
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SARS-CoV-2
/
COVID-19
Language:
English
Journal:
J. med. virol
Year:
2022
Document type:
Article
Institution/Affiliation country:
Ambulatório de Doenças Infecciosas/BR
/
Instituto Adolfo Lutz/BR
/
Instituto de Infectologia Emílio Ribas/BR