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Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
Aquino, Samuel Ricarte de; Diniz, Lucyo Flávio Bezerra; Nunes, Sávio Luiz Pereira; Silva, Roberta Lane de Oliveira; Gouveia, Gisele Veneroni; Gouveia, João José Simoni; Sales, Kamila Gaudêncio da Silva; Dantas-Torres, Filipe; Carmo, Rodrigo Feliciano do.
Affiliation
  • Aquino, Samuel Ricarte de; Universidade Federal do Vale do São Francisco. Programa de Pós-Graduação em Ciências da Saúde e Biológicas. Petrolina. BR
  • Diniz, Lucyo Flávio Bezerra; Universidade Federal do Vale do São Francisco. Programa de Pós-Graduação em Ciências da Saúde e Biológicas. Petrolina. BR
  • Nunes, Sávio Luiz Pereira; Universidade de Pernambuco. Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada. Recife. BR
  • Silva, Roberta Lane de Oliveira; Universidade Federal do Vale do São Francisco. Laboratório de Microbiologia Geral. Petrolina. BR
  • Gouveia, Gisele Veneroni; Universidade Federal do Vale do São Francisco. Colegiado de Zootecnia. Petrolina. BR
  • Gouveia, João José Simoni; Universidade Federal do Vale do São Francisco. Colegiado de Zootecnia. Petrolina. BR
  • Sales, Kamila Gaudêncio da Silva; Instituto Aggeu Magalhães. Departamento de Imunologia. Recife. BR
  • Dantas-Torres, Filipe; Instituto Aggeu Magalhães. Departamento de Imunologia. Recife. BR
  • Carmo, Rodrigo Feliciano do; Universidade Federal do Vale do São Francisco. Programa de Pós-Graduação em Ciências da Saúde e Biológicas. Petrolina. BR
Rev. Soc. Bras. Med. Trop ; 56: e0456, 2023. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1431406
Responsible library: BR1.1
ABSTRACT
ABSTRACT

Background:

This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.

Methods:

Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.

Results:

A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six.

Conclusions:

We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.

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Full text: Available Collection: International databases Health context: SDG3 - Health and Well-Being Health problem: Target 3.3: End transmission of communicable diseases Database: LILACS Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2023 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Instituto Aggeu Magalhães/BR / Universidade Federal do Vale do São Francisco/BR / Universidade de Pernambuco/BR
Fulltext
Add to My VHL
Print
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Full text: Available Collection: International databases Health context: SDG3 - Health and Well-Being Health problem: Target 3.3: End transmission of communicable diseases Database: LILACS Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2023 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Instituto Aggeu Magalhães/BR / Universidade Federal do Vale do São Francisco/BR / Universidade de Pernambuco/BR