Morphofunctional regeneration by mesenchymal stem cell and IGF-1 inoculation in a model of facial nerve crush injury in rats
Braz. j. otorhinolaryngol. (Impr.)
; 89(2): 244-253, March-Apr. 2023. tab, graf
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1439725
Responsible library:
BR1.1
ABSTRACT
Abstract Objective:
To analyze the morphofunctional regeneration process of facial nerve injury in the presence of insulin-like growth factor-1 and mesenchymal stem cells.Methods:
Fourteen Wistar rats suffered unilateral facial nerve crushing and were randomly divided into two groups. All received insulin-like growth factor-1 inoculation, but only half of the animals received an additional inoculation of mesenchymal stem cells. The animals were followed for 90 days and facial nerve regeneration was analyzed via spontaneous facial motor function tests and immunohistochemistry in the nerve motor nucleus.Results:
The group that received the growth factor and stem cells showed a statistically superior mean in vibrissae movements (p<0.01), touch reflex (p = 0.05) and eye closure (p<0.01), in addition to better immunohistochemistry reactivity. There was a statistically significant difference in the mean number of cells in the facial nerve nucleus between the experimental groups (p = 0.025), with the group that received the growth factor and stem cells showing the highest mean.Conclusion:
The association between growth factor and stem cells potentiates the morphofunctional regeneration of the facial nerve, occurring faster and more effectively. Level of Evidence 4, degree of recommendation C.
Full text:
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Collection:
International databases
Database:
LILACS
Type of study:
Prognostic study
Language:
English
Journal:
Braz. j. otorhinolaryngol. (Impr.)
Journal subject:
Otolaryngology
Year:
2023
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Norte/BR
/
Universidade do Estado do Rio Grande do Norte/BR