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Production, characterization and kinetic model of biosurfactant produced by lactic acid bacteria
Montoya Vallejo, Carolina; Flórez Restrepo, Maria Alejandra; Guzmán Duque, Fernando León; Quintero Díaz, Juan Carlos.
Affiliation
  • Montoya Vallejo, Carolina; Universidad de Antioquia. Departamento de Ingeniería Química. Antoquia. CO
  • Flórez Restrepo, Maria Alejandra; Universidad de Antioquia. Escuela de Microbiologia. Antioquia. CO
  • Guzmán Duque, Fernando León; Universidad de Antoquia. Escuela de Microbiologia. Antioquia. CO
  • Quintero Díaz, Juan Carlos; Universidad de Antioquia. Escuela de Microbiologia. Antioquia. CO
Electron. j. biotechnol ; 53: 14-22, Sep.2021. tab, graf
Article in English | LILACS | ID: biblio-1444470
Responsible library: CL61.1
ABSTRACT
BACKGROUND Biosurfactants are surface active molecules produced by microorganisms which have the ability to disrupt the plasma membrane. Biosurfactant properties are important in the food, pharmaceu tical and oil industries. Lactic acid bacteria can produce cell-bound and excreted biosurfactants. RESULTS The biosurfactant-producing ability of three Lactobacillus strains was analyzed, and the effects of carbon and nitrogen sources and aeration conditions were studied. The three species of lactobacillus eval uated were able to produce biosurfactants in anaerobic conditions, which was measured as the capacity of one extract to reduce the surface tension compared to a control. The decreasing order of biosurfactant production was L. plantarum>Lactobacillus sp.>L. acidophilus. Lactose was a better carbon source than glu cose, achieving a 23.8% reduction in surface tension versus 12.9% for glucose. Two complex nitrogen sources are required for growth and biosurfactant production. The maximum production was reached at 48 h under stationary conditions. However, the highest level of production occurred in the exponential phase. Biosurfactant exhibits a critical micelle concentration of 0.359 ± 0.001 g/L and a low toxicity against E. coli. Fourier transform infrared spectroscopy indicated a glycoprotein structure. Additionally, the kinetics of fermentation were modeled using a logistic model for the biomass and the product, achieving a good fit (R2 > 0.9). CONCLUSIONS L. plantarum derived biosurfactant production was enhanced using adequate carbon and nitrogen sources, the biosurfactant is complex in structure and because of its low toxicity could be applied to enhance cell permeability in E. coli
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Collection: International databases Database: LILACS Main subject: Lactic Acid / Lactobacillus plantarum Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Document type: Article Affiliation country: Colombia Institution/Affiliation country: Universidad de Antioquia/CO / Universidad de Antoquia/CO
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Collection: International databases Database: LILACS Main subject: Lactic Acid / Lactobacillus plantarum Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Document type: Article Affiliation country: Colombia Institution/Affiliation country: Universidad de Antioquia/CO / Universidad de Antoquia/CO
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