Primer design for complete sequencing of the Avastrovirus ORF2 gene / Desenho de primers para o sequenciamento completo do gene ORF2 em Avastrovirus
Semina ciênc. agrar
; 39(1): 411-418, jan.-fev. 2018. tab, ilus
Article
in En
| VETINDEX
| ID: biblio-1501065
Responsible library:
BR68.1
Localization: BR68.1
ABSTRACT
Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention.
RESUMO
As infecções por avastrovírus estão associados à doença entérica, nefrite e hepatite em aves. Aqui, nos presentamos um protocolo planejado para o sequenciamento completo do gene ORF2 em Avian Nephritis Vírus (ANV), Chicken Astrovirus (CAstV) e Turkey Astrovirus tipo 1 (TAstV-1), usando a técnica de sequenciamento convencional de Sanger. Foram usados primers previamente descritos e desenhados neste estudo, tendo como alvo as regiões conservadas flanqueadoras e internas dentro do gene ORF2 nos três vírus. O conhecimento destas sequencias é um elemento chave para caracterizar o vírus e prover de dados em diversos aspectos da epidemiologia e prevenção da doença.
Key words
Full text:
1
Database:
VETINDEX
Main subject:
Birds
/
Base Sequence
/
Polymerase Chain Reaction
/
Avastrovirus
/
Genes, Viral
Limits:
Animals
Language:
En
Journal:
Semina ciênc. agrar
Year:
2018
Document type:
Article