Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
Biol. Res
; 54: 6-6, 2021. ilus, graf, tab
Article
in English
| LILACS
| ID: biblio-1505798
Responsible library:
CL1.1
ABSTRACT
BACKGROUND:
Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear.RESULTS:
Cytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS.CONCLUSIONS:
Our data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Oryza
/
Electron Transport Complex I
/
Plant Infertility
/
Mitochondria
Type of study:
Risk factors
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2021
Document type:
Article
Affiliation country:
China
Institution/Affiliation country:
Guangxi Botanical Garden of Medicinal Plant/CN
/
Guangxi University/CN