Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis
Braz. oral res. (Online)
; 37: e115, 2023. tab, graf
Article
in English
|
LILACS-Express
| LILACS, BBO - Dentistry
| ID: biblio-1520525
Responsible library:
BR1.1
ABSTRACT
Abstract This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II - probiotic water without AP; Group III - regular water with AP; Group IV - probiotic water with AP. AP was induced after 3 days in the control groups and after 7, 21, and 42 days in groups III and IV. The animals were euthanized, and the mandibles were subjected to histotechnical processing. Samples were stained with hematoxylin & eosin (H&E) to identify root canal features, apical and periapical regions. Additionally, histoenzymology was performed to detect osteoclasts, immunohistochemistry was used to identify osteoclastogenesis markers, and the Brown & Brenn technique was applied for microbiological analysis. The data were analyzed using GraphPad Prism 8.0.1 with a significance level of 5%. Although no statistical differences were observed, the groups administered with probiotics showed better conditions in terms of histological aspects seen microscopically. Furthermore, there were no differences in the number of osteoclasts (p > 0.05). The RANKL marker was not found in the probiotic group at 42 days, unlike in group III.
Full text:
Available
Collection:
International databases
Database:
BBO - Dentistry
/
LILACS
Language:
English
Journal:
Braz. oral res. (Online)
Journal subject:
Dentistry
Year:
2023
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR