Drug-resistant polymorphisms and copy numbers in plasmodium falciparum, mozambique, 2015
Emerg. infect. dis. (Online)
; 24(1): 40-48, Jan. 2018. tab, graf, mapas
Article
in En
| AIM, RSDM
| ID: biblio-1527112
Responsible library:
MZ1.1
ABSTRACT
One of the fundamental steps toward malaria control is the use of antimalarial drugs. The success of antimalarial treatment can be affected by the presence of drug-resistant populations of Plasmodium falciparum. To assess resistance, we used molecular methods to examine 351 P. falciparum isolates collected from 4 sentinel sites in Mozambique for K13, pfmdr1, pfcrt, and pfdhps polymorphisms and for plasmepsin2 (pfpm2) and pfmdr1 copy numbers. We found multiple copies of pfpm2 in 1.1% of isolates. All isolates carried K13 wild-type alleles (3D7-like), except 4 novel polymorphisms (Leu619Leu, Phe656Ile, Val666Val, Gly690Gly). Prevalence of isolates with pfcrt mutant (K76T) allele was low (2.3%). Prevalence of isolates with pfdhps mutant alleles (A437G and K540E) was >80%, indicating persistence of sulfadoxine/pyrimethamine resistance; however, markers of artemisinin were absent, and markers of piperaquine resistance were low. Piperaquine resistance isolates may spread in Mozambique as dihydroartemisinin/piperaquine drug pressure increases.
Key words
Full text:
1
Collection:
06-national
/
MZ
Database:
AIM
/
RSDM
Main subject:
Malaria, Falciparum
/
DNA Copy Number Variations
Type of study:
Risk_factors_studies
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Country/Region as subject:
Africa
Language:
En
Journal:
Emerg. infect. dis. (Online)
Year:
2018
Document type:
Article