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The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT in oral squamous cell carcinoma
Ruiman, ZHOU; Zhong, CHEN; Yihuang, CAI; Huilian, ZHANG; Shunjie, MAO; Yunan, ZHUANG; Jiacheng, ZHENG.
Affiliation
  • Ruiman, ZHOU; Xiamen Medical College. Department of Stomotology. Xiamen. CN
  • Zhong, CHEN; Xiamen Medical College. Department of Stomotology. Xiamen. CN
  • Yihuang, CAI; Xiamen Medical College. Department of Stomotology. Xiamen. CN
  • Huilian, ZHANG; Xiamen Medical College. Department of Stomotology. Xiamen. CN
  • Shunjie, MAO; Xiamen Medical College. Department of Stomotology. Xiamen. CN
  • Yunan, ZHUANG; Xiamen Medical College. Department of Stomotology. Xiamen. CN
  • Jiacheng, ZHENG; Xiamen Medical College. Department of Stomotology. Xiamen. CN
J. appl. oral sci ; J. appl. oral sci;32: e20240215, 2024. graf
Article in En | LILACS-Express | LILACS | ID: biblio-1575148
Responsible library: BR1.1
ABSTRACT
Abstract Objective This study aims to explore the effects of miR-223-3p and miR-155-5p on epithelial-mesenchymal transition (EMT) and migration in oral squamous cell carcinoma (OSCC). Methodology EMT markers (E-cadherin, N-cadherin, P120 catenin (P120ctn), and vimentin) expression was determined by qRT-PCR and western blot analysis in SCC-9 cells which overexpress miR-155-5p and/or not express miR-223-3p. Scratch assays and Transwell migration assays were conducted to evaluate cell migration ability. Results When miR-223-3p was inhibited in OSCC cells, P120ctn and E-cadherin mRNA levels were dramatically downregulated (P<0.05), while N-cadherin levels were significantly upregulated, and the migration ability of OSCC cells increased. The overexpression of miR-155-5p in OSCC cells upregulated miR-223-3p significantly (34-fold) compared to the control group. It also led to significant downregulation of the mRNA of P120ctn and E-cadherin and significant upregulation of the mRNA of N-cadherin and Vimentin (P<0.05). Meanwhile, the migratory ability of OSCC cells significantly increased. When miR-155-5p was overexpressed while miR-223-3p was inhibited, the highest expression of E-cadherin and P120ctn mRNA and the lowest expression of N-cadherin(P<0.05) was observed. Simultaneously, tumor cell migration was significantly facilitated. Conclusion miR-223-3p inhibits the migration of OSCC cells, while miR-155-5p can elevate the miR-223-3p mRNA expression. The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT, increasing OSCC migration in vitro. This provides a novel approach and potential target for the effective treatment of OSCC.
Key words

Full text: 1 Collection: 01-internacional Database: LILACS Language: En Journal: J. appl. oral sci Journal subject: ODONTOLOGIA Year: 2024 Document type: Article / Project document Affiliation country: China Country of publication: Brazil

Full text: 1 Collection: 01-internacional Database: LILACS Language: En Journal: J. appl. oral sci Journal subject: ODONTOLOGIA Year: 2024 Document type: Article / Project document Affiliation country: China Country of publication: Brazil