Experimental model of glucocorticoid-induced insulin resistance
Acta cir. bras
; 31(10): 645-649, Oct. 2016. tab, graf
Article
in English
| LILACS
| ID: biblio-827650
Responsible library:
BR1.1
ABSTRACT
ABSTRACT PURPOSE:
To evaluate metabolic effects in experimental model of glucocorticoid-induced insulin resistance.METHODS:
Twenty Wistar male rats were randomly divided into two groups, which were treated with intraperitoneally injected dexamethasone 1mg/Kg/day for ten days consecutively (Group D; n=10) and placebo (Group C; n=10). The variables analyzed were from the first to the 10th day - body weight (before and after treatment); food and water daily consumption; on the 10th day - glycemia, insulinemia, HOMA-beta and HOMA-IR. The blood samples for laboratory analysis were obtained by intracardiac puncture. Also on the 10th day liver fragments were taken for analyzing glycogen and fattty.RESULTS:
Group D animals compared to group C had weight reduction (g), (D=226.5±24.7 vs C=295.0±25.4; p=0.001); increased glycemia (mmol/l) (D=19.5±2.1 vs C=14.2±3.1; p=0.0001); diminished insulinemia (mU/l) (D=0.2±0.1 vs C=2.0±0.4; p=0.0001); reduced HOMA-β (D=0.2±0.1 vs C=4.2±1.7; p=0.0002); diminished HOMA-IR (D=0.2±0.1 vs C=1.3±0.4; p=0.0002). Histological examination of the liver showed that 100% of group D and none of group C had moderate fatty. (p=0.2).CONCLUSION:
Animals treated with glucocorticoid, in this experimental model, expressed hyperglycemia, hypoinsulinism and decreased peripheral insulin sensitivity.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Insulin Resistance
/
Dexamethasone
/
Glucocorticoids
Type of study:
Evaluation study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2016
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade do Vale do Sapucaí/BR