Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
Braz. j. microbiol
; 48(2): 366-372, April.-June 2017. tab, graf
Article
in En
| LILACS
| ID: biblio-839381
Responsible library:
BR1.1
ABSTRACT
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Phylogeny
/
Molecular Diagnostic Techniques
/
Herpesviridae
/
Malignant Catarrh
Type of study:
Diagnostic_studies
/
Qualitative_research
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil