The protection effect and mechanism of hyperbaric oxygen therapy in rat brain with traumatic injury
Acta cir. bras
; 33(4): 341-353, Apr. 2018. graf
Article
in En
| LILACS
| ID: biblio-886284
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the effect of hyperbaric oxygen therapy (HBOT) on traumatic brain injury (TBI) outcome.Methods:
The modified Marmarou's weight drop device was used to generate non-lethal moderate TBI rat model, and further developed in vitro astrocytes culturing system. Then, we analyzed the expression changes of interested genes and protein by quantitative PCR and western blot.Results:
Multiple HBO treatments significantly reduced the expression of apoptosis promoting genes, such as c-fos, c-jun, Bax and weakened the activation of Caspase-3 in model rats. On the contrary, HBOT alleviated the decrease of anti-apoptosis gene Bcl-2 and promoted the expression of neurotrophic factors (NTFs), such as NGF, BDNF, GDNF and NT-3 in vivo. As a consequent, the neuropathogenesis was remarkably relied with HBOT. Astrocytes from TBI brain or those cultured with 21% O2 density expressed higher NTFs than that of corresponding controls, from sham brain and cultured with 7% O2, respectively. The NTFs expression was the highest in astrocytes form TBI brain and cultured with 21% O2, suggesting a synergistic effect existed between TBI and the following HBO treatment in astrocytes.Conclusion:
Our findings provided evidence for the clinical usage of HBO treating brain damages.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Brain Injuries, Traumatic
/
Hyperbaric Oxygenation
Type of study:
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Document type:
Article
Affiliation country:
China
Country of publication:
Brazil