Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
An. acad. bras. ciênc
; 90(1): 99-108, Mar. 2018. graf
Article
in English
| LILACS
| ID: biblio-886876
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Sulfhydryl Compounds
/
Cysteamine
/
Cystine
/
Disulfides
/
Homeostasis
/
Kidney
Type of study:
Evaluation study
Limits:
Animals
Language:
English
Journal:
An. acad. bras. ciênc
Journal subject:
Science
Year:
2018
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Sul/BR