An investigation of virulence factors of Legionella pneumophila environmental isolates
Braz. j. microbiol
; Braz. j. microbiol;49(1): 189-199, Jan.-Mar. 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-889209
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Bacterial Proteins
/
Legionella pneumophila
/
Virulence Factors
Limits:
Animals
/
Female
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2018
Document type:
Article
/
Project document
Affiliation country:
Turkey
Country of publication:
Brazil