Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells
J. appl. oral sci
; 25(6): 631-640, Nov.-Dec. 2017. tab, graf
Article
in En
| LILACS, BBO
| ID: biblio-893662
Responsible library:
BR1.1
ABSTRACT
Abstract Objectives:
The primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (TEGDMA) on odontoclastic differentiation in the dental pulp tissue. Material andMethods:
The effects of different TEGDMA dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies i) flow cytometry and tartrate-resistant acid phosphatase (TRAP) staining; ii) apoptotic effects using Annexin V staining; iii) mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF)-kB ligand (RANKL) genes by quantitative Real-time PCR (qRT-PCR); and iv) OPG and RANKL protein expression by enzyme-linked immunosorbent assay (ELISA).Results:
TEGDMA caused relatively less odontoclastic differentiation in comparison with the control group; however, odontoclastic differentiation augmented with increasing doses of TEGDMA (p<0.05). The mRNA and protein expression of OPG was lower in TEGDMA treated pulp cells than in the control group (p<0.05). While the mRNA expression of RANKL remained unchanged compared to the control group (p>0.05), its protein expression was higher than the control group (p<0.05). In addition, TEGDMA increased the apoptosis of dental pulp cells dose dependently.Conclusions:
TEGDMA reduced the odontoclastic differentiation ability of human dental pulp cells. However, odontoclastic differentiation ratios increased proportionally with the increasing dose of TEGDMA.Key words
Full text:
1
Collection:
01-internacional
Database:
BBO
/
LILACS
Main subject:
Polyethylene Glycols
/
Polymethacrylic Acids
/
Cell Differentiation
/
Dental Pulp
/
Tartrate-Resistant Acid Phosphatase
Limits:
Humans
Language:
En
Journal:
J. appl. oral sci
Journal subject:
ODONTOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
Turkey
Country of publication:
Brazil