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Comparative analysis of Mouse Inoculation Test and Virus Isolation in Cell Culture for rabies diagnosis in animals of Parana, Brazil
Corona, Thaila Francini; Böger, Beatriz; Rocha, Tatiana Carneiro da; Svoboda, Walfrido Külh; Gomes, Eliane Carneiro.
Affiliation
  • Corona, Thaila Francini; Laboratório Central do Estado do Paraná. Divisão de Epidemiologia e Laboratórios de Controle de Doenças. Divisão de Zoonoses. Curitiba. BR
  • Böger, Beatriz; Laboratório Central do Estado do Paraná. Divisão de Epidemiologia e Laboratórios de Controle de Doenças. Divisão de Zoonoses. Curitiba. BR
  • Rocha, Tatiana Carneiro da; Laboratório Central do Estado do Paraná. Divisão de Epidemiologia e Laboratórios de Controle de Doenças. Divisão de Zoonoses. Curitiba. BR
  • Svoboda, Walfrido Külh; Laboratório Central do Estado do Paraná. Divisão de Epidemiologia e Laboratórios de Controle de Doenças. Divisão de Zoonoses. Curitiba. BR
  • Gomes, Eliane Carneiro; Laboratório Central do Estado do Paraná. Divisão de Epidemiologia e Laboratórios de Controle de Doenças. Divisão de Zoonoses. Curitiba. BR
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;51(1): 39-43, Jan.-Feb. 2018. tab
Article in En | LILACS | ID: biblio-897046
Responsible library: BR1.1
ABSTRACT
Abstract

INTRODUCTION:

Rabies is an acute zoonotic disease, caused by a rhabdovirus that can affect all mammals, and is commonly transmitted by the bite of a rabid animal. The definitive diagnosis is laboratorial, by the Fluorescent Antibody Test (FAT) as a quick test and Mouse Inoculation Test (MIT) as a confirmatory test (gold standard). Studies conducted over the past three decades indicate that MIT and Virus Isolation in Cell Culture (VICC) can provide the same effectiveness, the latter being considered superior in bioethics and animal welfare. The aim of this study was to compare VICC with MIT, in terms of accuracy, biosafety and occupational health, supply and equipment costs, bioethics and animal welfare, in a Brazilian public health lab.

METHODS:

We utilized 400 samples of animal neurological tissue to compare the performance of VICC against MIT. The variables analyzed were accuracy, biosafety and occupational health, time spent in performing the tests, supply and equipment costs, bioethics and animal welfare evaluation.

RESULTS:

Both VICC and MIT had almost the same accuracy (99.8%), although VICC presented fewer risks regarding biosafety and mental health of the technicians, and reduced time between inoculation and obtaining the results (approximately 22 days less). In addition, VICC presented lower supply costs (86.5% less), equipment costs (32.6% less), and the advantage of not using animals.

CONCLUSIONS:

These results confirm that VICC can replace MIT, offering the same accuracy and better features regarding cost, results, biosafety and occupational health, and bioethics and animal welfare.
Subject(s)
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Rabies / Rabies virus / Occupational Health / Fluorescent Antibody Technique / Cell Culture Techniques / Containment of Biohazards / Bioethical Issues Type of study: Diagnostic_studies / Etiology_studies / Health_economic_evaluation Aspects: Determinantes_sociais_saude / Ethics Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Rev. Soc. Bras. Med. Trop Journal subject: MEDICINA TROPICAL Year: 2018 Document type: Article Affiliation country: Brazil Country of publication: Brazil

Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Rabies / Rabies virus / Occupational Health / Fluorescent Antibody Technique / Cell Culture Techniques / Containment of Biohazards / Bioethical Issues Type of study: Diagnostic_studies / Etiology_studies / Health_economic_evaluation Aspects: Determinantes_sociais_saude / Ethics Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Rev. Soc. Bras. Med. Trop Journal subject: MEDICINA TROPICAL Year: 2018 Document type: Article Affiliation country: Brazil Country of publication: Brazil