IL32 expression in peripheral blood CD3+ cells from myelodysplastic syndromes patients
Appl. cancer res
; 37: 1-6, 2017. tab, ilus
Article
in English
| LILACS, Inca
| ID: biblio-915122
Responsible library:
BR30.1
ABSTRACT
Background:
Myelodysplastic syndromes (MDS) are a heterogeneous group of disorders characterized by ineffective hematopoiesis and risk of leukemia transformation. There is evidence to suggest the participation of immune system deregulation in MDS pathogenesis. Interleukin-32 (IL-32) is a newly described multifunctional cytokine reported as an important mediator in autoimmune and inflammatory disorders. In the present study, we reported the expression of IL32 and IL32 transcript variants (α, ß, γ and δ) in peripheral blood CD3+ cells from healthy controls and MDS patients.Methods:
CD3+ cells were isolated by immunomagnetic cell sorting from thirty-nine untreated MDS patients and twenty-nine healthy donors. Gene expression was evaluated by quantitative PCR. For statistical analysis, MannWhitney test, Kruskal-Wallis test with Dunns post test and Log-rank (Mantel-Cox) were used, as appropriate. A p value <0.05 was considered statistically significant.Results:
IL32 expression and IL32 transcript variants IL32α, IL32ß, IL32γ, and IL32δ, were similar in peripheral blood CD3+ cells from healthy donors and MDS patients. Increased IL-32α expression was an independent predictor for MDS disease progression by univariate and multivariate analysis.Conclusions:
We observed that IL32 expression is not differently expressed in CD3+ cells from MDS patients; nevertheless IL32α has a potential role in disease progression (AU)
Full text:
Available
Collection:
International databases
Database:
LILACS
/
Inca
Main subject:
Myelodysplastic Syndromes
/
Multivariate Analysis
/
Interleukins
/
CD3 Complex
/
Disease Progression
/
Immune System
Type of study:
Prognostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
Appl. cancer res
Journal subject:
Neoplasms
Year:
2017
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of Campinas, Hemocentro-Unicamp/BR
/
University of São Paulo at Ribeirão Preto Medical School/BR