In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
Biol. Res
; 51: 12, 2018. graf
Article
in En
| LILACS
| ID: biblio-950898
Responsible library:
CL1.1
ABSTRACT
BACKGROUND:
Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the process of spermatogenesis. METHODS ANDRESULTS:
CCR6 was expressed in spermatogenic cell lines and its expression was shown in an age-dependent upregulation manner from puberty to adulthood in mouse testis. Immunostaining results confirmed the localization of CCR 6 in testis. Further chemotaxis assays demonstrated that spermatogenic cells GC-1 and -2 exhibited a directional movement toward CCR6-specific ligand such as CCL20 or Sertoli cells in vitro.CONCLUSIONS:
The present findings indicate that CCR6 is involved in the chemotaxis of spermatogenic cells in vitro and promotes chemotaxis under non-inflammatory conditions during normal spermatogenesis.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Spermatogenesis
/
Chemotaxis
/
Cryptorchidism
/
Chemokine CCL20
/
Receptors, CCR6
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
2018
Document type:
Article
Affiliation country:
China
Country of publication:
Chile