Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)
Braz. arch. biol. technol
; 59: e16150335, 2016. tab
Article
in English
| LILACS
| ID: biblio-951349
Responsible library:
BR1.1
ABSTRACT
ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the viability of the cells. It was concluded that "aroeira" extract was able to change the gingival fibroblast viability, and this effect was concentration dependent.
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Collection:
International databases
Database:
LILACS
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2016
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
USP/BR