Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
Rev. bras. farmacogn
; 28(4): 457-467, July-Aug. 2018. graf
Article
in English
| LILACS
| ID: biblio-958892
Responsible library:
BR1.1
ABSTRACT
Abstract Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew & R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 µM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells in sub-G1 phase, DNA internucleosomal fragmentation and activation of caspase-3. Western blot analysis revealed that zeaxanthin induced up-regulation of the pro-apoptotic factors Bim and Bid and inhibition of NF-κB transactivation. Additionally, zeaxanthin sensitized A2058 melanoma cells in vitro to the cytotoxic activity of vemurafenib, a BRAF inhibitor widely used for the clinical management of melanoma, suggesting its potential interest as dietary adjuvant increasing melanoma cells sensitivity to chemotherapy.
Full text:
Available
Collection:
International databases
Database:
LILACS
Type of study:
Prognostic study
Language:
English
Journal:
Rev. bras. farmacogn
Journal subject:
Pharmacy
Year:
2018
Document type:
Article
Affiliation country:
Brazil
/
France
Institution/Affiliation country:
Federal University of San Francisco Valley/BR
/
Institut Français de Recherche pour l'Exploitation de la Mer/FR
/
University of La Rochelle/FR