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Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
Khan, Anwar Sheed; Ali, Sajid; Khan, Muhammad Tahir; Ahmed, Sajjad; Khattak, Yasir; Abduljabbar,; Irfan, Muhammad; Sajjad, Wasim.
Affiliation
  • Khan, Anwar Sheed; Khyber Medical University Peshawar. Peshawar. PK
  • Ali, Sajid; Provincial TB Reference Laboratory. Peshawar. PK
  • Khan, Muhammad Tahir; Capital University of Science & Technology. Islamabad. PK
  • Ahmed, Sajjad; Khyber Medical University Peshawar. Peshawar. PK
  • Khattak, Yasir; University of Peshawar. Centre for Biotechnology and Microbiology. Peshawar. PK
  • Abduljabbar,; University of Haripur. Department of Medical Lab Technology. Haripur. PK
  • Irfan, Muhammad; Sarhad University of Science and Information Technology. Institute of Biological Sciences. Peshawar. PK
  • Sajjad, Wasim; National University of Medical Sciences. Department of Biogenetics. Rawalpindi. PK
Braz. j. microbiol ; Braz. j. microbiol;49(4): 909-913, Oct.-Dec. 2018. tab
Article in En | LILACS | ID: biblio-974301
Responsible library: BR1.1
ABSTRACT
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Tuberculosis, Pulmonary / Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Microscopy / Mycobacterium tuberculosis Type of study: Diagnostic_studies Limits: Adult / Female / Humans / Male Country/Region as subject: Asia Language: En Journal: Braz J Microbiol / Braz. j. microbiol / Braz. j. microbiol. (Online) / Brazilian journal of microbiology (Impresso) Journal subject: MICROBIOLOGIA Year: 2018 Document type: Article Affiliation country: Pakistan Country of publication: Brazil
Fulltext
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Tuberculosis, Pulmonary / Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Microscopy / Mycobacterium tuberculosis Type of study: Diagnostic_studies Limits: Adult / Female / Humans / Male Country/Region as subject: Asia Language: En Journal: Braz J Microbiol / Braz. j. microbiol / Braz. j. microbiol. (Online) / Brazilian journal of microbiology (Impresso) Journal subject: MICROBIOLOGIA Year: 2018 Document type: Article Affiliation country: Pakistan Country of publication: Brazil