Your browser doesn't support javascript.
loading
Endogenous ADP-ribosylation of eukaryotic elongation factor 2 and its 32 kDa tryptic fragment
Ergen, K; Bektas, M; G§kþe, S; Nurten, R.
Affiliation
  • Ergen, K; Kocaeli University Medical Faculty. Department of Biophysics. Izmit-Kocaeli. Turkey
  • Bektas, M; Kocaeli University Medical Faculty. Department of Biophysics. Izmit-Kocaeli. Turkey
  • G§kþe, S; Kocaeli University Medical Faculty. Department of Biophysics. Izmit-Kocaeli. Turkey
  • Nurten, R; Kocaeli University Medical Faculty. Department of Biophysics. Izmit-Kocaeli. Turkey
Biocell ; 31(1): 61-66, abr. 2007. ilus
Article in English | BINACIS | ID: bin-122869
Responsible library: AR40.1
ABSTRACT
Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis. In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related to protein synthesis. This modification appears to be important for the cell function.(AU)
Subject(s)
Full text: Available Collection: National databases / Argentina Database: BINACIS Main subject: Bacterial Toxins / Glycosylation / Adenosine Diphosphate Ribose / ADP Ribose Transferases Limits: Animals Language: English Journal: Biocell Year: 2007 Document type: Article Institution/Affiliation country: Kocaeli University Medical Faculty/Turkey
Full text: Available Collection: National databases / Argentina Database: BINACIS Main subject: Bacterial Toxins / Glycosylation / Adenosine Diphosphate Ribose / ADP Ribose Transferases Limits: Animals Language: English Journal: Biocell Year: 2007 Document type: Article Institution/Affiliation country: Kocaeli University Medical Faculty/Turkey
...