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Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
Rodriguez, Andrea P; Tsujigiwa, Hidetsugu; Gunduz, Mehmet; Cengiz, Beyhan; Nagai, Noriyuki; Tamamura, Ryo; Borkosky, Silvia S; Takagi, Tohru; Inoue, Miho; Nagatsuka, Hitoshi.
Affiliation
  • Rodriguez, Andrea P; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Tsujigiwa, Hidetsugu; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Gunduz, Mehmet; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Cengiz, Beyhan; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Nagai, Noriyuki; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Tamamura, Ryo; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Borkosky, Silvia S; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Takagi, Tohru; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Inoue, Miho; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
  • Nagatsuka, Hitoshi; Okayama University. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Department of Oral Pathology and Medicine. Okayama. Japan
Biocell ; 33(1): 39-47, Apr. 2009. ilus
Article in English | BINACIS | ID: bin-127215
Responsible library: AR40.1
ABSTRACT
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cel ls, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.(AU)
Subject(s)
Full text: Available Collection: National databases / Argentina Database: BINACIS Main subject: Sialoglycoproteins / Bone Regeneration / Collagen Type I / Odontoblasts Type of study: Prognostic study Limits: Animals Language: English Journal: Biocell Year: 2009 Document type: Article Institution/Affiliation country: Okayama University/Japan
Full text: Available Collection: National databases / Argentina Database: BINACIS Main subject: Sialoglycoproteins / Bone Regeneration / Collagen Type I / Odontoblasts Type of study: Prognostic study Limits: Animals Language: English Journal: Biocell Year: 2009 Document type: Article Institution/Affiliation country: Okayama University/Japan
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